Went camping this weekend and on the last day while packing the bike I realised the flat has returned.

After 3 hour drive home with the AA and replacing the tube I found a long nail in the tyre and it turns out the tube had green slime in it. That is why the first time it went flat the slime blocked the hole and after the two up ride this weekend it was punctured again but the slime did not work.

Lesson learned: Take spare tubes wherever you go.

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